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recombinant human il 21 miltenyi biotec  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec recombinant human il 21 miltenyi biotec
    Recombinant Human Il 21 Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+il+21/Human+IL-21%2C+research+grade/pm42486103-554-23-26
    Average 94 stars, based on 13 article reviews
    recombinant human il 21 miltenyi biotec - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Modification:

    Article Title: N 6 -methyladenosine RNA base modification regulates NKG2D-dependent and cytotoxic genes expression in natural killer cells
    Article Snippet: .. NK cells were cultivated in Iscove's Modified Dulbecco's Medium (IMDM) + 10% FCS + 1% Penicillin/Streptomycin and supplemented with irradiated K562-mbIL15-41BBL feeder cells, recombinant IL-2 (200 IU/ml, NIH Cytokine Repository, USA) and 100 ng/ml recombinant human IL-21 (Miltenyi, Germany). ..

    Article Title: N 6 -methyladenosine RNA base modification regulates NKG2D-dependent and cytotoxic genes expression in natural killer cells.
    Article Snippet: .. NK cells were cultivated in Iscove’s Modified Dulbecco’s Medium (IMDM) + 10% FCS + 1% Penicillin/ Streptomycin and supplemented with irradiated K562mbIL15-41BBL feeder cells, recombinant IL-2 (200 IU/ ml, NIH Cytokine Repository, USA) and 100 ng/ml recombinant human IL-21 (Miltenyi, Germany). ..

    Irradiation:

    Article Title: N 6 -methyladenosine RNA base modification regulates NKG2D-dependent and cytotoxic genes expression in natural killer cells
    Article Snippet: .. NK cells were cultivated in Iscove's Modified Dulbecco's Medium (IMDM) + 10% FCS + 1% Penicillin/Streptomycin and supplemented with irradiated K562-mbIL15-41BBL feeder cells, recombinant IL-2 (200 IU/ml, NIH Cytokine Repository, USA) and 100 ng/ml recombinant human IL-21 (Miltenyi, Germany). ..

    Article Title: N 6 -methyladenosine RNA base modification regulates NKG2D-dependent and cytotoxic genes expression in natural killer cells.
    Article Snippet: .. NK cells were cultivated in Iscove’s Modified Dulbecco’s Medium (IMDM) + 10% FCS + 1% Penicillin/ Streptomycin and supplemented with irradiated K562mbIL15-41BBL feeder cells, recombinant IL-2 (200 IU/ ml, NIH Cytokine Repository, USA) and 100 ng/ml recombinant human IL-21 (Miltenyi, Germany). ..

    Recombinant:

    Article Title: N 6 -methyladenosine RNA base modification regulates NKG2D-dependent and cytotoxic genes expression in natural killer cells
    Article Snippet: .. NK cells were cultivated in Iscove's Modified Dulbecco's Medium (IMDM) + 10% FCS + 1% Penicillin/Streptomycin and supplemented with irradiated K562-mbIL15-41BBL feeder cells, recombinant IL-2 (200 IU/ml, NIH Cytokine Repository, USA) and 100 ng/ml recombinant human IL-21 (Miltenyi, Germany). ..

    Article Title: Next‐generation Bruton's tyrosine kinase inhibitor BIIB091 selectively and potently inhibits B cell and Fc receptor signaling and downstream functions in B cells and myeloid cells
    Article Snippet: Untouched B cells were purified from healthy donor PBMCs using EasySep ® Human B Cell Enrichment Kit (STEMCELL Technologies) and labelled with CellTraceTM CFSE (Life Technologies, Carlsbad, CA). .. Labelled B cells were then stimulated with TLR9 ligand CpG ODN 2006 (Invivogen, San Diego, CA) or F(ab′)2 goat anti‐human IgM (1 μg mL −1 ; Jackson ImmunoResearch) or combinations of anti‐IgM)/MEGACD40L (1 μg mL −1 ; Enzo Life Sciences, Farmingdale, NY) with either recombinant human IL‐4 (20 ng mL −1 ; R&D Systems) or recombinant human IL‐21 (20 ng mL −1 ; Miltenyi Biotec) in the presence or absence of 100 n m of BIIB091 in serum‐free X‐VIVO 10TM medium for 3–5 days. ..

    Article Title: Nucleofection-based screening of chimeric antigen receptor candidates in human natural killer cells
    Article Snippet: The remaining CD3 - NK cells were cultured at a density of 2 × 10 6 cells/ml in cBiotarget medium supplemented with 50 IU/ml IL-2 and 10 ng/ml IL-15 (STEMCELL Technologies #78031.2) until the day of electroporation. .. PBMCs (STEMCELL Technologies) were similarly thawed as described above in cRPMI media (Gibco #11875-093) and activated in the presence of bead-bound anti-CD3 and anti-CD28 antibodies (Gibco #11132D), 20 U/ml recombinant human IL-7 (Miltenyi Biotec #130-095-362), 10 U/ml recombinant human IL-15 (Miltenyi Biotec #130-095-764) and 0.04 U/ml recombinant human IL-21 (Miltenyi Biotec #130-095-769) for 3 days. ..

    Article Title: N 6 -methyladenosine RNA base modification regulates NKG2D-dependent and cytotoxic genes expression in natural killer cells.
    Article Snippet: .. NK cells were cultivated in Iscove’s Modified Dulbecco’s Medium (IMDM) + 10% FCS + 1% Penicillin/ Streptomycin and supplemented with irradiated K562mbIL15-41BBL feeder cells, recombinant IL-2 (200 IU/ ml, NIH Cytokine Repository, USA) and 100 ng/ml recombinant human IL-21 (Miltenyi, Germany). ..

    Article Title: From byproduct to biotherapeutic: Comparative study of buffy coats and leukoreduction system chambers for NK cell-based immunotherapies
    Article Snippet: PROLEUKIN®- Aldesleukin (IL-2) , Clinigen , Cat# PL31644/0003. .. Recombinant human IL-21 , Miltenyi Biotec , Cat# 130-095-767. .. Recombinant human IL-15 , Miltenyi Biotec , Cat# 130-095-760.

    Article Title: Nucleofection-based screening of chimeric antigen receptor candidates in human natural killer cells.
    Article Snippet: The remaining CD3- NK cells were cultured at a density of 2 × 106 cells/ml in cBiotarget medium supplemented with 50 IU/ml IL-2 and 10 ng/ml IL-15 (STEMCELL Technologies #78031.2) until the day of electroporation. .. PBMCs (STEMCELL Technologies) were similarly thawed as described above in cRPMI media (Gibco #11875-093) and activated in the presence of bead-bound anti-CD3 and anti-CD28 antibodies (Gibco #11132D), 20 U/ml recombinant human IL-7 (Miltenyi Biotec #130-095-362), 10 U/ml recombinant human IL-15 (Miltenyi Biotec #130-095-764) and 0.04 U/ml recombinant human IL-21 (Miltenyi Biotec #130-095-769) for 3 days. ..

    other:

    Article Title: Next-generation Bruton's tyrosine kinase inhibitor BIIB091 selectively and potently inhibits B cell and Fc receptor signaling and downstream functions in B cells and myeloid cells.
    Article Snippet: The IC was prepared by incubating equal volumes of human anti-RNP (RayBiotech Inc., Peachtree Corners, GA), isolated from SLE patient serum and purified RNP antigen (Arotec Diagnostics, Lower Hutt, New Zealand) at 37°C for 30 min.

    Article Title: IgA-Producing B Cells Exert Regulatory Function Through Granzyme B in Kidney Allograft Tolerance
    Article Snippet: B cells were purified from PBMCs of healthy blood donors by negative selection using Human B Cell Isolation Kit II and autoMACS PRO Separator, with purity >95% (Miltenyi Biotech, Gladbach, Germany), thereafter CD3- CD14- IgA+/- B cells were sorted on an ARIA III flow cytometry cell sorter (BD Biosciences, Le Pont-de-Claix, France).



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